Anti lamin a antibody c 3 is recommended for detection of lamin a of mouse rat and human origin by wb ip if and elisa.
Anti lamin a antibody.
Anti lamin a c terminal antibody is suitable for use in western blot 0 1 0 2 μg ml using hela nuclear extract.
Since both dna blue and lamin a c red are associated with the nuclear compartment this region appears crimson in this image.
The anti lamin a c antibody reveals strong nuclear lamina staining while anti lamp1 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes.
During apoptosis lamin a c is specifically cleaved into a large 41 50 kda and a small 28 kda fragment 3 4.
In humans this protein is encoded by the gene lmna.
See machiels et al 1997.
As a result this antibody recognizes lamin a but not lamin c.
The full protein is reported to be 664 amino acid residues in length.
Lamin a c antibodies.
74 kda observed band size.
It is listed to be a member of the intermediate filament family.
The protein may also be known as lamin c dhe lamin a cdcd1 cddc and cmd1a.
This antibody can also be used in indirect immunofluorescence 1 2 μg ml using hela cells rat nrk and mouse 3t3 cells.
Lamin a is a reported synonym of the human protein lamin a c encoded by the gene lmna.
Specific for an epitope mapping between amino acids 580 601 near the c terminus of lamin a of human origin.
The cells were labeled with lamin a monoclonal antibody 133a2 product ma1 06101 at 5 µg ml in 0 1 bsa and incubated overnight at 4 degree and then labeled with goat anti mouse igg h l superclonal secondary antibody alexa fluor 488 conjugate product a28175 at a dilution of 1 2000 for 45 minutes at room temperature panel a.
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope.
2000 j struct biol 129 313 23.
Anti lamin a c antibodies are available from several suppliers.
Additionally anti lamin a c terminal antibodies are suitable for use in immunoblotting approx.
Lamin a c is cleaved by caspase 6 and serves as a marker for caspase 6 activation.
Why is the actual band size different from the predicted.
Reacts against lamin a exclusively the antibody was raised against the carboxy terminus of 98 amino acids present in lamin a and absent from lamin c.
The cleavage of lamins results in nuclear dysregulation and cell death 5 6.
Anti lamin a antibody ab26300 at 1 1000 dilution hela whole cell extract at 100 µg secondary goat anti rabbit igg h l hrp conjugate at 1 10000 dilution developed using the ecl technique.
Anti lamin a antibodies are readily available from several suppliers.
The expected protein mass is 74 1 kda but there are 6 reported isoforms.
